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当前位置: 首页 > 产品中心 > Rabbit Monoclonal > Revmab/Biotin Anti-Human IgM Rabbit Monoclonal Antibody, Clone RM121/31-1023-02/1 Ea

Revmab/Biotin Anti-Human IgM Rabbit Monoclonal Antibody, Clone RM121/31-1023-02/1 Ea

Revmab/Biotin Anti-Human IgM Rabbit Monoclonal Antibody, Clone RM121/31-1023-02/1 Ea
  • Revmab/Biotin Anti-Human IgM Rabbit Monoclonal Antibody, Clone RM121/31-1023-02/1 Ea
商品介绍
ProductDetails
ProductNameBiotinAnti-HumanIgMRabbitMonoclonalAntibody[RM121]
ProductDescriptionBiotinylatedRabbitmonoclonaltoHumanIgM
CatalogNo.31-1023-02
CloneNameRM121
ProductDataSheetBiotinAnti-HumanIgMRabbitMonoclonalAntibodyDataSheetRM121
SpecificityRM121reactstotheheavychainofhumanIgM.NocrossreactivitywithhumanIgG,IgA,IgD,orIgE.
ImmunogenHumanIgM
ApplicationImmunohistochemistry(IHC)
FlowCytometry(FC)
Immunocytochemistry(ICC)
ELISA
SpeciesReactivityHuman
ConjugateBiotin
IntendedUseResearchUseOnly
.
Properties
FormLiquid
StorageInstructionsStoreat-20.0°C
StABIlityStablefor1Yearat-20.0°Cfromdateofreceipt
StorageBuffer50%Glycerol/PBSwith1%BSAand0.09%sodiumazide
Volume/ItemSize50ug
Concentration1.0mg/mL
PurityProteinAaffinitypurifiedfromananimalorigin–freeculturesupernatant
CloneTypeMonoclonal
IsotypeRabbitIgG
UsageELISA:50ng/well–200ng/well(forCapture);
0.05ug/mL–0.2ug/mL(forDetection);
Immunocytochemistry(ICC):0.5ug/mL-2ug/mL;
Immunohistochemistry(IHC):0.5ug/mL-2ug/mL.
Keywords
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ProductImages
Sandwich

SandwichELISAusingRM121asthecaptureantibody
(100ng/well), and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody, followed
by an alkaline phosphatase conjugated streptavidin.

Sandwich

SandwichELISAusingRM121asthecaptureantibody
(100ng/well), and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody, followed
by an alkaline phosphatase conjugated streptavidin.

ELISA

ELISAofhumanimmunoglobulinsshowsRM121
reacts only to human IgM. No cross reactivity
with Human IgG, IgE, IgD, or IgA. The plate was
coated with 50 ng/well of different immunoglobulins.
200 ng/mL, 50 ng/mL of RM121 was used as
the primary antibody. An alkaline phosphatase
conjugated anti-rabbit IgG as the secondary
antibody.

A

AtiterELISAusingRM121.Theplatewascoated
with different amounts of human IgM. A serial dilution
of RM121 was used as the primary antibody. An alkaline
phosphatase conjugated anti-rabbit IgG as the secondary
antibody.

品牌介绍

RevMAb Biosciences USA, Inc has developed a revolutionary monoclonal antibody generation method which allows development of monoclonal antibodies without generation of hybridoma. The recombinant antibodies are developed from a broad range of antigen-specific B cells that are inaccessible to traditional hybridoma technology. This revolutionary method has successfully been applied to develop high quality recombinant rabbit and goat monoclonal antibodies.

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