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当前位置: 首页 > 产品中心 > Rabbit Monoclonal > Revmab/Biotin Anti-His-Tag Rabbit Monoclonal Antibody, Clone RM146; 6x His-Tag or 10x His-Tag/31-1048-02/1 Ea

Revmab/Biotin Anti-His-Tag Rabbit Monoclonal Antibody, Clone RM146; 6x His-Tag or 10x His-Tag/31-1048-02/1 Ea

Revmab/Biotin Anti-His-Tag Rabbit Monoclonal Antibody, Clone RM146; 6x His-Tag or 10x His-Tag/31-1048-02/1 Ea
  • Revmab/Biotin Anti-His-Tag Rabbit Monoclonal Antibody, Clone RM146; 6x His-Tag or 10x His-Tag/31-1048-02/1 Ea
商品介绍
ProductDetails
ProductNameBiotinAnti-His-Tag(6xHis-Tagor10xHis-Tag)RabbitMonoclonalAntibody[RM146]
ProductDescriptionBiotinylatedRabbitmonoclonaltoHis-Tag.(6xHis-Tagor10xHis-Tag)ateithertheN-Terminus(amino)orC-Terminus(carboxy)
CatalogNo.31-1048-02
CloneNameRM146
ProductDataSheetBiotinAnti-HisTagRabbitMonoclonalAntibodyDataSheetRM146
SpecificityRM146reactstorecombinantproteinscontainingthe6xHis-Tagor10xHis-Tagfusedtoeithertheaminoorcarboxyterminus.Nocrossreactivitywithotherendogenousproteininmammalianorbacteriacells.
ImmunogenMixtureofapeptidewith6xHis-TagattheN-terminusandanotherpeptidewith6xHis-TagattheC-terminus.
ApplicationWesternBlot
ELISA
Immunoprecipitation
Immunocytochemistry
FlowCytometry
Immunohistochemistry
SpeciesReactivityAll
ConjugateBiotin
IntendedUseResearchUseOnly
.
Properties
FormLiquid
StorageInstructionsStoreat-20.0°C
StABIlityStablefor1Yearat-20.0°Cfromdateofreceipt
StorageBuffer50%Glycerol/PBSwith1%BSAand0.09%sodiumazide
Volume/Size50ug
Concentration1.0mg/mL
PurityProteinAaffinitypurifiedfromananimalorigin–freeculturesupernatant
CloneTypeMonoclonal
IsotypeRabbitIgG
UsageWesternBlot:0.1ug/mL–0.5ug/mL;
Immunocytochemistry0.5ug/mL–2ug/mL;
FlowCytometry:0.5ug/mL–2ug/mL;
Immunohistochemistry;0.1ug/mL-1ug/mL;
ELISA:0.01ug/mL-0.5ug/mL.
Keywords
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ProductImages
A

AtiterELISAperformedonpurifiedHisTag-PCSK9
recombinant protein. A serial dilution of Biotin Anti-
HisTag Rabbit Monoclonal Antibody Clone RM146
was used as the primary antibody, followed by
alkaline phosphatase conjugated Streptavidin.

Western

Westernblotof293Tcellstransfected(+)oruntrans-
fected (-) with a DNA construct encoding His-Tag
Histone H3 (G34W) protein, using Biotin Anti-HisTag
Rabbit Monoclonal Antibody Clone RM146 at 0.2 ug/mL,
followed by a HRP conjugated Streptavidin.

Immunohistochemistry

ImmunohistochemistrystainingofnaïveHepG2cells
(Negative control) using anti-His-Tag antibody, RM146.

Immunohistochemistry

Immunohistochemistrystainingof293Tcellsexpressing
His-Tag nuclear protein X, using anti-His-Tag antibody,
RM146.

品牌介绍

RevMAb Biosciences USA, Inc has developed a revolutionary monoclonal antibody generation method which allows development of monoclonal antibodies without generation of hybridoma. The recombinant antibodies are developed from a broad range of antigen-specific B cells that are inaccessible to traditional hybridoma technology. This revolutionary method has successfully been applied to develop high quality recombinant rabbit and goat monoclonal antibodies.

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