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Revmab/Anti-Mouse IgG2c Rabbit Monoclonal Antibody, Clone RM223/31-1103-00/1 Ea

Revmab/Anti-Mouse IgG2c Rabbit Monoclonal Antibody, Clone RM223/31-1103-00/1 Ea
  • Revmab/Anti-Mouse IgG2c Rabbit Monoclonal Antibody, Clone RM223/31-1103-00/1 Ea
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品牌介绍

RevMAb Biosciences USA, Inc has developed a revolutionary monoclonal antibody generation method which allows development of monoclonal antibodies without generation of hybridoma. The recombinant antibodies are developed from a broad range of antigen-specific B cells that are inaccessible to traditional hybridoma technology. This revolutionary method has successfully been applied to develop high quality recombinant rabbit and goat monoclonal antibodies.

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ProductDetails
ProductNameAnti-MouseIgG2cRabbitMonoclonalAntibody[RM223]
ProductDescriptionRabbitmonoclonaltoMouseIgG2c
CatalogNo.31-1103-00
CloneNameRM223
ProductDataSheetAnti-MouseIgG2cRabbitMonoclonalAntibodyDataSheetRM223
SpecificityRM223reactstotheFcregionofmouseIgG2c.NocrossreactivitywithmouseIgG1,IgG2a,IgG2b,IgG3,IgM,IgA,IgE,humanIgG,orratIgG.
ImmunogenMouseIgG2c
ApplicationWesternBlot(WB)(nonreducedonly)
Immunoprecipitation(IP)
Immunohistochemistry(IHC)
FlowCytometry(FC)
Immunocytochemistry(ICC)
ELISA
SpeciesReactivityMouse
ConjugateNone
IntendedUseResearchUseOnly
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Properties
FormLiquid
StorageInstructionsStoreat-20.0°C
StABIlityStablefor1Yearat-20.0°Cfromdateofreceipt
StorageBuffer50%Glycerol/PBSwith1%BSAand0.09%sodiumazide
Volume/ItemSize100ug
Concentration1.0mg/mL
PurityProteinAaffinitypurifiedfromananimalorigin–freeculturesupernatant
CloneTypeMonoclonal
IsotypeRabbitIgG
UsageELISA:0.01ug/mL–0.2ug/mL
Immunocytochemistry(ICC):0.5ug/mL-2ug/mL
Immunohistochemistry(IHC):0.5ug/mL-2ug/mL
WesternBlot(WB):0.5ug/mL-2ug/mL
Keywords
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ProductImages
ELISA

ELISAofIgGfromBalB/CandC57BL6showsRM223
reacts to C57BL6 IgG containing IgG2c, and does not
react to BalB/C IgG containing IgG2a. 200 ng/mL, 50
ng/mL, or 10 ng/mL of RM223 was used as the primary
antibody. An alkaline phosphatase conjugated anti-rabbit
IgG as the secondary antibody.

A

AtiterELISAofmouseIgG2c.Theplatewas
coated with different amounts of mouse IgG2c. A
serial dilution of RM223 was used as the primary
antibody. An alkaline phosphatase conjugated
anti-rabbit IgG as the secondary antibody.

Western

Westernblotofnonreduced(-)andreduced(+)
mouse IgG2c, using 0.5ug/mL of Revmabclone
RM223. This antibody reacts to nonreduced
IgG2c