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Revmab/Anti-Human IgG4 Rabbit Monoclonal Antibody, Clone RM120/31-1022-00/1 Ea

Revmab/Anti-Human IgG4 Rabbit Monoclonal Antibody, Clone RM120/31-1022-00/1 Ea
  • Revmab/Anti-Human IgG4 Rabbit Monoclonal Antibody, Clone RM120/31-1022-00/1 Ea
商品介绍
ProductDetails
ProductNameAnti-HumanIgG4RabbitMonoclonalAntibody[RM120]
ProductDescriptionRabbitmonoclonaltoHumanIgG4
CatalogNo.31-1022-00
CloneNameRM120
ProductDataSheetAnti-HumanIgG4RabbitMonoclonalAntibodyDataSheetRM120
SpecificityRM120reactstotheheavychainofhumanIgG4,RM120doesnotcrossreacttoanyotherIgGsubclasses(IgG1,IgG2,orIgG3),andshowsnocrossreactivitytoIgM,IgA,IgD,IgE.RM120doesnotreacttomonkey(CynoorRhesus)IgG,mouseIgG,ratIgG,orgoatIgG.
ImmunogenPeptidecorrespondingtothehingeregionofHumanIgG4
ApplicationImmunohistochemistry(IHC)
FlowCytometry(FC)
Immunocytochemistry(ICC)
ELISA
WesternBlot
SpeciesReactivityHuman
ConjugateNone
IntendedUseResearchUseOnly
.
Properties
FormLiquid
StorageInstructionsStoreat-20.0°C
StABIlityStablefor1Yearat-20.0°Cfromdateofreceipt
StorageBuffer50%Glycerol/PBSwith1%BSAand0.09%sodiumazide
Volume/ItemSize100ug
Concentration1.0mg/mL
PurityProteinAaffinitypurifiedfromananimalorigin–freeculturesupernatant
CloneTypeMonoclonal
IsotypeRabbitIgG
UsageELISA:50ng/well–200ng/well(forCapture);
0.05ug/mL–0.2ug/mL(forDetection);
WesternBlot(WB):0.5ug/mL–2ug/mL;
Immunocytochemistry(ICC):1ug/mL-10ug/mL;
Immunohistochemistry(IHC):1ug/mL-10ug/mL.
Keywords
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ProductImages
Detection

DetectionofhumanIgG4inmonkeyserum.
Sandwich ELISA using RM120 as the capture
antibody, and biotinylated anti-human light
chains ( κ+ λ) antibody RM129 as the detection
antibody, followed by a HRP conjugated streptavidin.

Sandwich

SandwichELISAusingRM120asthecapture
antibody, and biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody,
followed by an AP conjugated streptavidin.

Western

WesternBlotofhuman,mouse,rat,andgoat
IgG shows RM120 reacts to human IgG4, in
both whole molecule (~150kDa, non-reduced)
and heavy chain (~50kDa, reduced) forms. No
cross reactivity with other isotypes of human IgG,
or mouse, rat, or goat IgG.

A

AtiterELISAusingRM120.Theplatewascoated
with different amounts of human IgG4. A serial dilution
of RM120 was used as the primary antibody, followed
by an alkaline phosphatase conjugated anti-rabbit IgG
as the secondary antibody.

Immunohistochemistry

Immunohistochemicalstainingofformalinfixed
and paraffin embedded human lymphoid tissue
section using anti-human IgG4 rabbit monoclonal
antibody (Clone RM120).

ELISA

ELISAshowingRM120doesnotreacttomonkey
IgG. The plate was coated with Rhesus monkey IgG.
A serial dilution of RM120 and a monkey IgG binding
antibody (positive control) was used as the
detection antibody.

品牌介绍

RevMAb Biosciences USA, Inc has developed a revolutionary monoclonal antibody generation method which allows development of monoclonal antibodies without generation of hybridoma. The recombinant antibodies are developed from a broad range of antigen-specific B cells that are inaccessible to traditional hybridoma technology. This revolutionary method has successfully been applied to develop high quality recombinant rabbit and goat monoclonal antibodies.

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