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当前位置: 首页 > 产品中心 > Rabbit Monoclonal > Revmab/Anti-Dimethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody, Clone RM135; H3K4me2, Histone H3 (dimethyl K4)/31-1037-00/

Revmab/Anti-Dimethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody, Clone RM135; H3K4me2, Histone H3 (dimethyl K4)/31-1037-00/

Revmab/Anti-Dimethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody, Clone RM135; H3K4me2, Histone H3 (dimethyl K4)/31-1037-00/
  • Revmab/Anti-Dimethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody, Clone RM135; H3K4me2, Histone H3 (dimethyl K4)/31-1037-00/
商品介绍
ProductDetails
ProductNameAnti-Dimethyl-HistoneH3(Lys4)RabbitMonoclonalAntibody[RM135]
ProductDescriptionRabbitmonoclonaltoDimethylatedHistoneH3Lysine4,H3K4me2,HistoneH3(dimethylK4)
CatalogNo.31-1037-00
CloneNameRM135
ProductDataSheetAnti-Dimethyl-HistoneH3(Lys4)RabbitMonoclonalAntibodyDataSheetRM135
SpecificityRM135reactstoHistoneH3dimethylatedatLysine4(K4me2).NocrossreactivitywithmonomethylatedLysine4(K4me1),trimethylatedLysine4(K4me3),orothermethylationsinhistoneH3.
ImmunogenAdimethyl-peptidecorrespondingtoDimethyl-HistoneH3(Lys4).
ApplicationWesternBlot(WB)
Immunocytochemistry(ICC)
ELISA
Multiplex
SpeciesReactivityHuman
Vertebrates
ConjugateNone
IntendedUseResearchUseOnly
.
Properties
FormLiquid
StorageInstructionsStoreat-20.0°C
StABIlityStablefor1Yearat-20.0°Cfromdateofreceipt
StorageBuffer50%Glycerol/PBSwith1%BSAand0.09%sodiumazide
Volume/Size100ug
Concentration1.0mg/mL
PurityProteinAaffinitypurifiedfromananimalorigin–freeculturesupernatant
CloneTypeMonoclonal
IsotypeRabbitIgG
UsageWesternBlot:0.02ug/mL–0.2ug/mL;
ICC:1ug/mL-2ug/mL;
ELISA:0.2ug/mL-1ug/mL;
Multiplex:0.1ug/mL–0.5ug/mL.

Keywords
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ProductImages
RM135

RM135specificallyreactstoHistoneH3dimethylated
at Lysine 4 (K4me2). No cross reactivity with mono-
methylated Lysine 4 (K4me1), trimethylated Lysine 4
(K4me3), or other methylations in histone H3.

Western

WesternBlotofrecombinanthistoneH3.3(1)and
acid extracts of HeLa cells (2), using RM135 at 0.025
ug/mL, showed a band of histone H3 dimethylated
at Lysine 4 (K4me2) in HeLa cells.

Immunocytochemistry

ImmunocytochemistryofHeLacellstreatedwith
sodium butyrate, using Dimethyl-Histone H3 (Lys4)
Rabbit mAb RM135 (red). Actin filaments have
been labeled with fluorescein phalloidin (green).

品牌介绍

RevMAb Biosciences USA, Inc has developed a revolutionary monoclonal antibody generation method which allows development of monoclonal antibodies without generation of hybridoma. The recombinant antibodies are developed from a broad range of antigen-specific B cells that are inaccessible to traditional hybridoma technology. This revolutionary method has successfully been applied to develop high quality recombinant rabbit and goat monoclonal antibodies.

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