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当前位置: 首页 > 产品中心 > Rabbit Monoclonal > Revmab/Anti-Acetyl-Histone H3 (Lys36) Rabbit Monoclonal Antibody, Clone RM154; H3K36ac, Histone H3 (K36 acetylation)/31-1050-

Revmab/Anti-Acetyl-Histone H3 (Lys36) Rabbit Monoclonal Antibody, Clone RM154; H3K36ac, Histone H3 (K36 acetylation)/31-1050-

Revmab/Anti-Acetyl-Histone H3 (Lys36) Rabbit Monoclonal Antibody, Clone RM154; H3K36ac, Histone H3 (K36 acetylation)/31-1050-
  • Revmab/Anti-Acetyl-Histone H3 (Lys36) Rabbit Monoclonal Antibody, Clone RM154; H3K36ac, Histone H3 (K36 acetylation)/31-1050-
商品介绍
ProductDetails
ProductNameAnti-Acetyl-HistoneH3(Lys36)RabbitMonoclonalAntibody[RM154]
ProductDescriptionRabbitmonoclonaltoAcetylatedHistoneH3Lysine36(K36ac),H3K36ac,HistoneH3(K36acetylation)
CatalogNo.31-1050-00
CloneNameRM154
ProductDataSheetAnti-Acetyl-HistoneH3Lys36RabbitMonoclonalAntibodyDataSheetRM154
SpecificityRM154reactstoHistoneH3acetylatedatLysine36(K36ac),andisnotaffectedbythephosphorylationofneighboringSer31.NocrossreactivitywithotheracetylatedLysinesinhistoneH3.
ImmunogenAnacetyl-peptidecorrespondingtoAcetyl-HistoneH3(Lys36).
ApplicationWesternBlot(WB)
Immunocytochemistry(ICC)
ELISA
Multiplex
SpeciesReactivityHuman
Vertebrates
ConjugateNone
IntendedUseResearchUseOnly
.
Properties
FormLiquid
StorageInstructionsStoreat-20.0°C
StABIlityStablefor1Yearat-20.0°Cfromdateofreceipt
StorageBuffer50%Glycerol/PBSwith1%BSAand0.09%sodiumazide
Volume/Size100ug
Concentration1.0mg/mL
PurityProteinAaffinitypurifiedfromananimalorigin–freeculturesupernatant
CloneTypeMonoclonal
IsotypeRabbitIgG
UsageWesternBlot:0.5ug/mL-2ug/mL;
ICC:0.5ug/mL-2ug/mL;
ELISA:0.2ug/mL-1ug/mL;
Multiplex:0.1ug/mL–0.5ug/mL.
Keywords
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ProductImages
RM154

RM154specificallyreactstoHistoneH3acetylated
at Lysine 36 (K36ac), and is not affected by the
phosphorylation of neighboring Ser31. No cross
reactivity with acetylated Lysine 4 (K4ac), Lysine 9
(K9ac), Lysine 14 (K14ac), Lysine 18 (K18ac),
Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 56
(K56ac), lysine 79 (K79ac), or Lysine 122 (K122)
in histone H3.

Western

WesternBlotofacidextractsfromHeLacells
untreated (-) or treated with sodium butyrate (+),
using RM154 at 1.0 ug/mL, showed a band of
histone H3 acetylated at Lysine 36 in treated
HeLa.

Immunocytochemistry

ImmunocytochemistryofHeLacellstreatedwith
sodium butyrate, using Acetyl-Histone H3 (Lys36)
Rabbit mAb RM154 (red). Actin filaments have
been labeled with fluorescein phalloidin (green).

品牌介绍

RevMAb Biosciences USA, Inc has developed a revolutionary monoclonal antibody generation method which allows development of monoclonal antibodies without generation of hybridoma. The recombinant antibodies are developed from a broad range of antigen-specific B cells that are inaccessible to traditional hybridoma technology. This revolutionary method has successfully been applied to develop high quality recombinant rabbit and goat monoclonal antibodies.

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